Carnosine decreases PMA-induced oxidative stress and inflammation in murine macrophages
Laboratory studyOther animals
- Design
- Laboratory study
- Subjects
- not stated in abstract (cell culture, RAW 264.7 murine macrophages)
- Dose used in the study
- up to 20 mM carnosine in culture medium (highest dose stated)
- Duration
- not stated in abstract
- What was measured
- Intracellular superoxide, Nox1 and Nox2 gene expression, Akt phosphorylation, cytokine mRNA (TNF-alpha, IL-6, IL-4, IL-10, TGF-beta1), nucleoside triphosphates, NADP+/NADPH ratio in macrophages challenged with PMA and superoxide dismutase inhibitors.
What the authors reported
The authors report that carnosine lowered intracellular superoxide and reduced Nox1 and Nox2 expression, and attenuated PMA-induced Akt phosphorylation. It reduced TNF-alpha and IL-6 mRNA and increased IL-4, IL-10 and TGF-beta1 mRNA. At 20 mM it raised total nucleoside triphosphates and the pool of nicotinic coenzymes, and it decreased the NADP+/NADPH ratio in a dose-dependent manner. The authors conclude that carnosine acts through several mechanisms in macrophages under oxidative and inflammatory stress.
Limits of this study
In-vitro study in a mouse macrophage cell line; concentrations used (up to 20 mM) are laboratory concentrations, not dietary exposures.
Source
PubMed 31390749 · doi:10.3390/antiox8080281
Entry checked against full text (PubMed Central, PMC6720685) on 2026-10-11. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.