Carnosine prevents the glycation-induced changes in electrophoretic mobility of aspartate aminotransferase

Seidler NW.. Journal of Biochemical and Molecular Toxicology, 2000; 14(4):215-220.

Laboratory studyOther animals

Design

Laboratory study

Dose used in the study

1 to 20 mM carnosine

Duration

4 days at 37 degrees C

What was measured

Electrophoretic mobility of purified cardiac aspartate aminotransferase (2 mg/mL) incubated with 500 micromolar glyceraldehyde 3-phosphate with or without carnosine.

What the authors reported

Carnosine at carnosine-to-glyceraldehyde 3-phosphate ratios of 10:1 or higher prevented glycation-induced modification of the enzyme. The modified form's apparent molecular weight shifted after two days, which the author interprets as rearrangement of the initial adduct.

Limits of this study

Cell-free in vitro system. Full text not read.

Source

PubMed 10789500 · doi:10.1002/(SICI)1099-0461(2000)14:4<215::AID-JBT6>3.0.CO;2-Z

Entry checked against PubMed abstract (E-utilities) on 2026-10-19. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.