Retardation of the senescence of cultured human diploid fibroblasts by carnosine
Laboratory studyOther animals
- Design
- Laboratory study
- Dose used in the study
- 20 to 50 mM carnosine in culture medium
- What was measured
- Growth, morphology and lifespan (population doublings) of cultured human diploid fibroblasts (HFF-1 foreskin and MRC-5 fetal lung cells) grown with or without carnosine.
What the authors reported
Carnosine at 20 to 50 mM retarded senescence and rejuvenated senescent cultures; late-passage cells kept a non-senescent morphology while carnosine was present and reverted to the senescent phenotype when moved to normal medium. Serial subculture in carnosine did not remove the Hayflick limit, although lifespan in population doublings and in chronological age was often increased. D-carnosine, homocarnosine, anserine and beta-alanine did not have the same effects. The authors suggest carnosine preserves cellular integrity through effects on protein metabolism rather than antioxidant action alone.
Limits of this study
Cell culture at concentrations far above those reached in human plasma. The lifespan extension was variable and obscured by normal variability of fibroblast lifespans. Abstract only.
Source
PubMed 8187813 · doi:10.1006/excr.1994.1132
Entry checked against PubMed abstract (E-utilities) on 2026-10-19. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.