Analysis of an H1 receptor-mediated, zinc-potentiated vasoconstrictor action of the histidyl dipeptide carnosine in rabbit saphenous vein
Laboratory studyOther animals
- Design
- Laboratory study
- Dose used in the study
- carnosine in rings up to 10 mM; binding assay with zinc at 10, 30 and 80 microM
- What was measured
- Contraction of isolated rabbit saphenous vein rings and [3H]-mepyramine binding to H1 sites in guinea-pig cerebellar membranes.
What the authors reported
Mepyramine antagonised carnosine-induced contractures (Schild pA2 7.97) and histamine-induced contractures (pA2 8.48). Histamine contractures were smaller (73%) and less well sustained than carnosine contractures. Serotonergic antagonists methiothepin and ketanserin also antagonised both, which the authors read as coincidental H1 inhibition. With zinc present, carnosine inhibited [3H]-mepyramine binding to guinea-pig cerebellar membranes (log IC50 -2.78, -3.93 and -4.64 at 10, 30 and 80 microM zinc, corrected for zinc's own inhibition). Carnosine also evoked an H2-mediated relaxation in the presence of mepyramine. The authors conclude carnosine can act at the smooth muscle H1 receptor.
Limits of this study
Isolated tissue; n not stated in abstract. Full text not read.
Source
PubMed 9863657 · doi:10.1038/sj.bjp.0702184
Entry checked against PubMed abstract (E-utilities) on 2026-10-19. The dose shown is the dose the researchers used. It is not a recommendation. How to read this page.